McIntosh Lab Publications


Ras signal requires dynamic properties of Ets1 for phosphorylation-enhanced binding to co-activator CBP. Nelson, M.L., Kang, H.-S., Lee, G.M., Blaszczak, A.G., Lau, D.K.W., McIntosh, L.P., and Graves, B.J. Proc. Natl. Acad. Sci. 107: 10026-10031 (2010). (suppl material)

DNA binding by the ETS protein TEL (ETV6) is regulated by autoinhibition and self-association. Green, S.M., Coyne III, H.J., McIntosh, L.P., and Graves, B.J. J. Biol. Chem. 285: 18496-18504 (2010). (suppl material)

The anti-invasive peptide Neopetrosiamide A induces the release of membrane-bound vesicles and inhibits tumor cell adhesion. Austin, P., Heller, M., Williams, D.E., McIntosh, L.P., Vogl, W., Foster, L.J., Andersen, R.J., Roberge, M., and Roskelly, C.D. P.L.O.S. (in press 2010).

Circular permutation of Bacillus circulans xylanase: a kinetic and structural study. Reitinger, S., Yu, Y., Wicki, J., Ludwiczek, M., D’Angelo, I., Baturin, S.J., Okon, M., Strynadka, N.C.J., Lutz, S., Withers, S.G., and McIntosh, L.P. Biochemistry 49: 2464-2474 (2010).

Titration_DB: Storage and analysis of NMR-monitored protein pH titration curvess. Farrell, D., Saja-Miranda, E., Webb, H., Georgi, N., Crowley, P., McIntosh, L.P. and Nielsen, J.E. Proteins 78: 843-857 (2010). (suppl material)


NMR Spectroscopic Characterization of the Sialyltransferase CstII from Camplyobacter jejuni: Histidine 188 is the General Base. Chan, P.H.W., Lairson, L.L., Lee, H.J., Wakarchuk, W.W., Strynadka, N.C.J., Withers, S.G., and McIntosh, L.P. Biochemistry 48: 11220-11230 (2009).

Detection and assignment of phosphoserine and phosphothreonine residues by 13C-31P spin-echo difference NMR spectroscopy. McIntosh, L.P., Kang, H.-S., Okon, M., Nelson, M.L., Graves, B.J., and Brutscher, B. J. Biomol. NMR 43: 31-37 (2009).


Structural characterization of the type III pilotin-secretin complex from Shigella flexneri. Okon, M., Moraes, T., Lario, P.I., Creagh, A. L., Haynes, C. A., Strynadka, N.C.J., and McIntosh, L.P. Structure 16: 1544-1554 (2008). (suppl material)

The crystal structure of MexR from Pseudomonas aeruginosa in complex with its anti-repressor ArmR. Wilke, M., Heller, M., Creagh, A.L., Haynes, C.A., McIntosh, L.P., Poole, K., and Strynadka, N.C.J. Proc. Natl. Acad. Sci. 105: 14831-14837 (2008). (suppl material)

The affinity of Ets-1 for DNA is modulated by phosphorylation through transient interactions of an unstructured region. Lee, G.M., Pufall, M.A., Meeker, C.A,, Kang, H.S., Graves, B.J., and McIntosh, L.P. J. Mol. Biol. 382: 1014-1030 (2008). (suppl material)

Phage display and crystallographic analysis reveals potential substrate / binding site interactions in the protein secretion chaperone CsaA from Agrobacterium tumefaciens. Feldman, A.R., Shapova, Y.A., Wu, S.S.T., Oliver, D.C., Heller, M., McIntosh, L.P., Scott, J.K., and Paetzel, M. J. Mol. Biol. 379: 457-470 (2008). (suppl material)

Identification and structural characterization of a CBP/p300-binding domain from the ETS family transcription factor GABPa. Kang, H.-S., Nelson, M.L. Mackereth, C.D., Schärpf, M., Graves B.J., and McIntosh, L.P. J. Mol. Biol. 377: 636-646 (2008). (suppl material)

Determination of electrostatic interaction energies and protonation state populations in enzyme active sites. Søndergaard, C. R., McIntosh, L.P., Pollastri, G., and Nielsen, J.E. J. Mol. Biol. 376: 269-287. (suppl material)


A secondary xylan binding site enhances the catalytic activity of a single domain family 11 glycoside hydrolase. Ludwiczek, M.L., Heller, M., Kantner, T., and McIntosh, L.P. J. Mol. Biol. 373: 337-354 (2007). (suppl. material)

Peptide binding by a fragment of calmodulin composed of EF-hands 2 and 3. Lakowski, T.M., Lee, G.M., Lelj-Garolla, B., Okon, M., Reid, R.E., and McIntosh, L.P. Biochemistry 46: 8525-8536 (2007).

Probing electrostatic interactions along the reaction pathway of a glycoside hydrolase: Histidine characterization by NMR spectroscopy. Schubert, M., Poon, D.K.Y., Wicki, J., Tarling, C.A., Kwan, E.M., Nielsen, J.E., Withers, S.G., and McIntosh, L.P. Biochemistry 46: 7383-7395 (2007). (suppl. material).

Calcium-induced folding of a fragment of calmodulin composed of EF-hands 2 and 3. Lakowski, T.M., Lee, G.M., Okon, M., Reid, R.E., and McIntosh, L.P. Prot. Sci. 16: 1119-1132 (2007).

Direct demonstration of the flexibility of the glycosylated proline-threonine linker in the Cellulomonas fimi xylanase Cex through NMR spectroscopic analysis. Poon, D.K.Y., Withers, S.G. and McIntosh, L.P. J. Biol. Chem. 282: 2091-2100 (2007). (suppl. material).

NMR spectroscopic characterization of a beta-(1,4)-glycosidase along its reaction pathway: stabilization upon formation of the glycosyl-enzyme intermediate. Poon, D.K.Y., Ludwiczek, M.L., Schubert, M., Kwan, E.M., Withers, S.G. and McIntosh, L.P. Biochemistry 46: 1759-1770(2006). (suppl. material).


Unambiguous determination of the ionization state of a glycoside hydrolase active site lysine by 1H-15N heteronuclear correlation spectroscopy, Poon, D.K.Y., Schubert, M., Au, J., Okon, M., Withers, S.G. and McIntosh, L.P. J.A.C.S. 128: 15388-15389 (2006). (suppl. material).

Beads-on-a-String, characterization of Ets-1 sumoylated within its flexible N-terminal sequence. Macauley, M.S., Errington, W.J., Scharpf, M., Mackereth, C.D., Blaszczak, A.G., Graves, B.J., and McIntosh, L.P. J. Biol. Chem. 281: 4164-4172 (2006). (suppl. material).

Expanding the thioglycoligase strategy to the synthesis of alpha-linked thioglycosidase allows the structural investigation of the parent enzyme/substrate complex. Kim, Y.-W., Lovering, A.L., Chen, H., Kantner, T., McIntosh, L.P., Strynadka, N.C.J., and Withers, S.G. J. Am. Chem. Soc. 128: 2202-2203 (2006). (suppl. material).

Glycosynthase-based synthesis of xylo-oligosaccharides using an engineered retaining xylanase from Cellulomonas fimi. Kim, Y.-W., Fox, D.T., Hekmat, O., Kantner, T., McIntosh, L.P., Warren, R.A.J., and Withers, S.G. Org. Biomol. Chem. 4: 2025-2032 (2006).


Dissecting the domain structure of Cdc4p, a mysoin essential light chain involved in Schizosaccharomyces pombe cytokinesis. Escobar-Cabera E., Venkatesan, M., Desaytels, M., Hemmingsen, S.M., McIntosh, L.P. Biochemistry 44: 12136-12148 (2005). (suppl. material).

Variable control of Ets-1 DNA binding by multiple phosphates in an unstructured region. Pufall, M.A., Lee, G.M., Nelson, M.L., Kang, H.-S., Velyvis, A., Kay, L.E., McIntosh, L.P., and Graves, B.J. Science 309: 142-145 (2005). (suppl. material).

The structural and dynamic basis of Ets-1 DNA binding autoinhibition. Lee, G.M., Donaldson, L.W., Pufall, M.A., Kang, H.-S., Pot, I., Graves, B.J., and McIntosh, L.P. J. Biol. Chem. 280: 7088-7099 (2005). (suppl. material).

Backbone chemical shift assignments of the LexA catalytic domain in its active conformation. Okon, M., Pfuetzner, R.A., Vuckovic, M., Little, J.W., Strynadka, N.C.J., and McIntosh, L.P. J. Biomol. NMR, 31: 371-372 (2005).


Cyclic enterobacterial common antigen: Potential contaminant of bacterially expresed protein preparations. Erbel, P.J.A., Seidel, R., MacIntosh, S.E., Gentile, L.N., Amor, J.C., Kahn, R.A., Prestegard, J.H., McIntosh, L.P., and Gardner, K.H. J. Biomol. NMR 29: 199-204 (2004).

Mutations in the SAM domain of the ETV6-NTRK3 chimeric tyrosine kinase block polymerization and transformation activity. Tognon, C.E., Mackereth, C.D., Somasiri, A.M., McIntosh, L.P., and Sorensen, P.H.B. Mol. Cell. Biol. 24: 4636-4650 (2004).

Structural characterization of the RNase E S1 domain and identification of its oligonucleotide-binding and dimerization interfaces. Schubert, M., Edge, R.E., Lario, P., Cook, M.A., Strynadka, N.C.J., Mackie, G.A., and McIntosh, L.P. J. Mol. Biol. 341: 37-54 (2004). (suppl. material).

Diversity in structure and function of Ets family PNT domains. Mackereth, C.D., Scharpf, M., Gentile, L.N., MacIntosh, S.E., Slupsky, C.M., and McIntosh, L.P. J. Mol. Biol. 342: 1249-1264 (2004). (suppl. material).

Structural and dynamic independence of isopeptide-linked RanGAP1 and SUMO-1. Macauley, M.S., Errington, W.J., Okon, M., Scharpf, M., Mackereth, C.D., Schulman, B.A., and McIntosh, L.P. J. Biol. Chem. 279: 49131-49137 (2004). (suppl. material).


Characterizing the pH-dependent Stability and Catalytic Mechanism of the Family 11 Xylanase from the Alkalophilic Bacillus agaradhaerens. D.K.Y. Poon, P. Webster, S.G. Withers and L.P. McIntosh. Carb. Res. 338: 415-421 (2003).


Mechanism of E47-Pip Interaction on DNA Resulting in Transcriptional Synergy and Activation of Immunoglobulin Germline Sterile Transcripts. S. Nagulapalli, A. Goheer, L. Pitt, L.P. McIntosh and M.L. Atchison. Mol. Cell. Biol. 22: 7337-7350 (2002)

Chemical shift and secondary structure conservation of the PNT/SAM domains from the Ets family of transcription factors. C.D. Mackereth, M. Schärpf, L.N. Gentile and L. P. McIntosh J. Biomol. NMR 24: 71-72 (2002).

Oligomerization-dependent association of the SAM domains from Schizosaccharomyces pombe Byr2 and Ste4. R. Ramachander, C.A. Kim, M.L. Phillips, C.D. Mackereth, C.D. Thanos, L.P. McIntosh and J.U. Bowie. J. Biol. Chem. 277: 39585-39593 (2002).

Site-Specific Characterization of the Association of Xylooligosaccharides with the CMB13 Lectin-Like Xylan-Binding Domain from Streptomyces lividans Xylanase 10A by NMR Spectroscopy. M. Schärpf, G. P. Connelly, G. M. Lee, A. B. Boraston, R. A. Warren and L. P. McIntosh Biochemistry 41: 4255-4263 (2002).

An NMR Approach to Structural Proteomics. A. Yee, X. Chang, A. Pineda-Lucenda, B. Wu, A. Semesi, B. Le, T. Ramelot, G. M. Lee, S. Bhattacharyya, P. Guttierez, A. Denisov, C.-H. Lee, J. Cort, G. Koslov, J. Liao, L. Chen, D. Wishart, W. Lee, L. P. McIntosh, K. Gehring, M.A. Kennedy, A. M. Edwards, and C. H. Arrowsmith, Proc. Natl. Acad. Sci. 99: 1825-1830 (2002).

The Solution Structure and Interactions of CheW form Thermotoga Maritima. I. J. Griswold, H. Zhou, R. V. Swanson, L. P. McIntosh, M. I. Simon, and F.W. Dahlquist. Nature Struct. Biol. 9:121-125 (2002).

Autoinhibition allosterically regulates Ets-1 DNA binding through a dipole driven phosphate contact. H. Wang, L. P. McIntosh, and B. J. Graves, J. Biol.Chem. 277: 2225-2233 (2002).


NMR-based structure of the conserved protein MTH865 from the Archaeon Methanobacterium thermoautotrophicum. G.M. Lee, A.M. Edwards, C.H. Arrowsmith, and L.P. McIntosh J. Biomol. NMR 21: 63-66 (2001) (suppl. material).

Assignment of selectively 13C-labeled cellopentaose synthesized using an enginered glycosynthase. E. Brun, H. Brumer III, L.F. MacKenzie, S. G. Withers, and L.P. McIntosh J. Biomol. NMR 21: 67-68 (2001).

Dissecting the electrostatic interactions and pH-dependent activity of a Family 11 Glycosidase. M.D. Joshi, G. Sidhu, J.E. Nielsen, G.D. Brayer, S.G. Withers, and L.P. McIntosh Biochemistry 40, 10115-10139 (2001) (suppl. material).

Structure of Cdc4p, a contractile ring protein essential for cytokinesis in Schizosaccharomyces pombe. C.M. Slupsky, M. Desautels, T. Huebert, R. Zhao, S.M. Hemmingsen and L.P. McIntosh. J. Biol. Chem. 276, 5943-5951 (2001).

Cdc4p, a contractile ring protein essential for cytokinesis in Schizosaccharomyces pombe, interacts with a phosphatylinositol 4-kinase. M. Desautels, J.P. Den Haese, C.M. Slupsky, L.P. McIntosh and S.M. Hemmingsen. J. Biol. Chem. 276, 5932-5942 (2001).


Structural proteomics of an archeon. D. Christendat, A. Yee, A. Dharamsi, Y. Kluger, A. Savchenko, J.R. Cort, V. Booth, C.D. Mackereth, V. Saridakis, I. Ekiel, G. Kozlov, K.L. Maxwell, N. Wu, L.P. McIntosh, K. Gehring, M.A. Kennedy, A.R. Davidson, E.F. Pai, M. Gerstein, A.M. Edwards and C.H. Arrowsmith. Nature Struct. Biol. 7, 903-909 (2000).

Zinc-bundle structure of the essential RNA polymerase subunit RPB10 from Methanobacterium thermoautotrophicum. C.D. Mackereth, C.H. Arrowsmith, A.M. Edwards and L.P. McIntosh. Proc. Natl. Acad. Sci. USA 97(12) 6316-6321 (2000)

Hydrogen bonding and catalysis: A novel explanation for how a single amino acid substitution can change the pH optimum of a glycosidase. M.D. oshi, G. Sidhu, I. Pot, G.D. Brayer, S.G. Withers and L.P. McIntosh J. Mol. Biol. 299, 255-279 (2000)

Structure and binding specificity of the second N-terminal cellulose-binding domain from Cellulomonas fimi endoglucanaseC. E. Brun, P.E. Johnson, A.L. Creagh, P. Tomme, P. Webster, C.A. Haynes and L.P. McIntosh, Biochemistry 39, 2445-2458 (2000) (suppl. material)

Binding site analysis of cellulose-binding domain CBD_N1 from endoglucanase C of Cellulomonas fimi by site-directed mutagenesis. J. Kormos, P.E. Johnson, E. Brun, P. Tomme, L.P. McIntosh C.A. Haynes and D.G. Kilburn, Biochemistry 39, 8844-8852 (2000)

Analysis of the dynamic properties of Bacillus circulans xylanase upon formation of a covalent glycosyl-enzyme intermediate. G.P. Connelly, S.G. Withers and L.P. McIntosh, Protein Science 9, 512-524 (2000)


The highly conserved beta-haripin of the paired DNA-binding domains is required for the assembly of Pax:Ets ternary complexes. W. Wheat, H. Lennox, D. Fitzsimmons, S.J. Krautkramer, L.N. Gentile, L.P. McIntosh and J. Hagman, Mol. Cell. Biol. 19, 2231-2241 (1999)

The cellulose-binding domains from Cellulomonas fimi CenC beta-1,4-glucanase bind nitroxide spin-labeled cellooligosaccharides in multiple orientations. P.E. Johnson, E. Brun, L. Mackenzie, S.G. Withers and L.P. McIntosh. J. Mol. Biol. 287, 609-625 (1999) (suppl. material)

Sugar ring distortion in the glycosyl-enzyme intermediate of a family G/11 xylanase. G. Sidhu, S.G. Withers, N.T. Nguyen, L.P. McIntosh, L. Ziser and G.D. Brayer, Biochemistry 38, 5346-5354 (1999)


Characterization of a buried neutral histidine in Bacillus circulans xylanase: internal dynamics and interaction with a bound water molecule, G.P. Connelly and L.P. McIntosh, Biochemistry 37, 1810-1818 (1998)

Binding and stablility of the N1 cellulose-binding domain of Cellulomonas fimi endoglucanase CenC, A.L. Creagh, J. Koska, P.E. Johnson, P. Tomme, M.D. Joshi, L.P. McIntosh, D.G. Kilburn and C.A. Haynes, Biochemistry 37, 3529-3535 (1998)

Assigning the NMR spectra of aromatic amino acids in proteins: analysis of two Ets pointed domains. C.M.Slupsky, L.N. Gentile and L.P. McIntosh, Biochemistry and Cell Biology (Thematic Issue on Biomedical NMR in Canada) 76, 379-390 (1998)

Calcium binding by The N-terminal cellulose-binding domain from Cellulomonas fimi beta-1,4-glucanase CenC. P.E. Johnson, A.L. Creagh, E. Brun, K. Joe, P. Tomme, C.A. Haynes and L.P. McIntosh, Biochemistry 37, 12772-12781 (1998)

Structure of the Ets-1 Pointed domain and MAP kinase phosphorylation site. C.M. Slupsky, L.N. Gentile, L.W. Donaldson, C.D. Mackereth, J.J. Seidel, B.J. Graves, and L.P. McIntosh, Proc. Natl. Acad. Sci. USA 95, 12129-12134 (1998)


Stereospecific assignments of the NH2 resonances from the primary amides of asparagine and glutamine side-chains in isotopically labeled proteins, L.P. McIntosh, E. Brun and L.E.J. Kay, Biomol. NMR 9, 306-312 (1997)

High Resolution NMR structure and backbone dynamics of the Bacillus subtilius response regulator, Spo0F. V.A. Feher, J.W. Zapf, J.A. Hoch, J.M. Whiteley, L.P. McIntosh, M. Rance, N.J. Skelton, F.W. Dahlquist, J. Cavanagh, Biochemistry, 36, 10015-10025 (1997)

Specific 15N, NH correlations for residues in 15N, 13C fractionally deuterated proteins that immediately follow methyl-containing amino acids, D.R. Muhandiram, P.E. Johnson, D. Yang, O. Zhang, L.P. McIntosh and L.E. Kay, J. Biomol. NM. 10, 283-288 (1997)

Complete measurement of the pKa values of the carboxyl and imidazole groups in Bacillus circulans xylanase. M.D. Joshi, A. Hedberg and L.P. McIntosh, Protein Science 6, 2667-2670 (1997)


Solution structure of the murine Ets-1 ETS domain: a winged helix-turn-helix DNA binding protein. L.W. Donaldson, J.P. Petersen, B.J. Graves and L.P. McIntosh, EMBO J. 15, 125-134 (1996)

Structural coupling of the inhibitory regions flanking the ETS domain of murine Ets-1. J.J. Skalicky, L.W. Donaldson, J.M. Petersen, B.J. Graves and L.P. McIntosh, Protein Science 5, 296-309 (1996)

The pKa of the general acid/base carboxyl group of a glycosidase cycles during catalysis: a 13C-NMR study of Bacillus circulans xylanase. L.P. McIntosh, G. Hand, P.E. Johnson, M.D. Joshi, M. Körner, L.A. Plesniak, L. Ziser, W.W. Wakarchuk and S.G. Withers, Biochemistry 35, 9958-9966 (1996)

Interaction of soluble celloliogosaccharides with the N-terminal cellulose-binding domain of Cellulomonas fimi CenC. 2. NMR and ultraviolet absorbance spectroscopy P.E. Johnson, P. Tomme, M.D. Joshi and L.P. McIntosh, Biochemistry 35, 13895-13906 (1996)

Secondary Structure and NMR Assignments of Bacillus circulans xylanase. L.A. Plesniak, W.W. Wakarchuk and L.P. McIntosh, Protein Science 5, 1118-1135 (1996)

Characterization of a buried neutral histidine in Bacillus circulans xylanase: NMR assignment, pH titration, and hydrogen exchange. L.A. Plesniak, G.P. Connelly, W.W. Wakarchuk and L.P. McIntosh, Protein Science 5, 2319-2328 (1996)

Structure of the N-terminal cellulose-binding domain of Cellulomonas fimi CenC determined by NMR spectroscopy. P.E. Johnson, M.D. Joshi, P. Tomme, D.G. Kilburn and L.P. McIntosh, Biochemistry 35, 14381-14394 (1996) (suppl. material)

ETS Domain DNA Binding. B.J. Graves, M.E. Gillespie and L.P. McIntosh, Nature (Scientific Correspondence) 384, 322 (1996)

Probing the Role of Tryptophan Residues in a Cellulose-Binding Protein by Chemical Modifications. M.R, Bray, P.E. Johnson, N.R. Gilkes, L.P. McIntosh, D.G. Kilburn, and R.A. Warren, Protein Science 5, 2311-2318 (1996)


Modulation of transcription factor Ets-1 DNA binding: DNA-induced unfolding of an alpha-helix. J.P. Petersen, J.J. Skalicky, L.W. Donaldson, L.P. McIntosh, T. Alber and B.J. Graves, Science 269,1866-1869 (1995)

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